Directed evolution is an essential part of successful strain engineering. We fine tune key parameters of chemical and physical mutagen exposure for each strain, from common cell factories such as E. coli and S. cerevisiae to physiologically most complex metabolite producing organisms (e.g. actinomycetes, Myxobacteria). To assure fast progress and optimal use of resources, we constantly optimize high-throughput screening conditions, achieving a fine balance between robustness of cultivation in μl-ml volumes and correlation with industrial-scale conditions.
Based on our in-depth knowledge of microbial physiology and biosynthetic pathways, insight from key analytical data and “omics” analyses, we conceptualize a comprehensive metabolic engineering or synthetic biology strategy for each project. We complement standard cloning procedures with DNA synthesis, strain-specific genetic tools, and a platform in collaboration with international organizations for cloning fragments of up to several kb of DNA. We can work on host strains made available by our clients or partners for heterologous expression of polyketides, peptides and proteins.
Manufacturing of Bio-products
Initiating the development of downstream processing steps early on in development of bioprocess technologies is one of the key advantages of our system. Our expertise ranges from simplest inactivated biomass-based products to multi-step isolation and purification of target molecules with Active Pharmaceutical Ingredient (API) grade purity. We always aim for the right balance between purity of the material and acceptable price of the final product, based on the requirements of each market segment.
Down Stream Process (DSP) development is always done with industrial scale in mind, taking into account the equipment and expertise available in partner’s production facilities. Diverse centrifugation or filtration-based approaches for biomass separation are followed by solid-liquid and/or liquid-liquid extractions and finally, in most demanding applications, by classical chromatography or preparative HPLC. Downstream development is fully supported by a wide range of readily available analytics.
To fully explore the potential of strains, bioprocess steps are developed carefully. Optimal media ingredients are selected based on detailed understanding of strains’ physiological parameters and using production partner’s existing sources wherever possible. With scale-up starting from 5L, and increasing through 30L to 150L bioreactors, we ensure stepwise optimization of key bioprocess parameters and finally facilitate smooth bioprocess transfer to production site.
Analysis of key parameters is established and constraints of downstream processing steps are taken into account. We incorporate innovative, economy inspired solutions to achieve maximum eco-efficiency. We provide our production partners and clients with confidence of achieving optimal use of resources and minimizing manufacturing costs of target products.
Biotech Division of CDigital is actively pursuing to develop indigenous expertise and commercial usage of new research in the areas of Synthetic Biotechnology, Microbial Biotechnology and Bio chemicals. We are committed to striving for building a strong footprint of biotechnology manufacturing in our country. Our prime focus is to help local businesses and research institutions in developing an ecosystem for biotechnology.
Production and Design development
CDigital, in collaboration with international partners, offer services in design, development and implementation of biotechnology solutions. Along with the production of advanced biofuels, bio-products, renewable chemicals, bio-based specialty chemicals (pharmaceutical intermediates, fine chemicals, food ingredients). Microbial design and analysis for production of enzymes, chemicals, polymers and natural products. Apart from design, we conduct technical studies and commercialization of biotech related research.
Technical services and Solutions
We provide affordable next-generation biotechnology solutions for agro-genomics, sequencing, food testing, soil fertility, water cleaning, industrial wastewater and effluents treatment, etc. We also deal in supplying technology platforms, equipment, scanners, analytical instruments, software, kits and reagents for biotechnology labs and manufacturing plants.
We are capable of rapidly setting up and achieving advancement towards improved yield and reduced level of impurities. In addition to profound understanding of natural product biosynthesis, we also surpass in development of highly efficient isolation and purification procedures. From pharmaceutically relevant APIs to vitamins and food colorants, CDigital partnering with international organizations works on strain and fermentation development in the natural products segment. Apart from this, our team works to scale up the fermentation process that produces cost effective and quick results through a robust manufacturing plant that meets its commercial objectives. We work on a variety of strains to offer customized solutions based on customer preference along with manufacturing bio-products of our interest.